Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Differently PEGylated Polymer Nanoparticles for Pancreatic Cancer Delivery: Using a Novel Near-Infrared Emissive and Biodegradable Polymer as the Fluorescence Tracer

doi: 10.3389/fbioe.2021.699610

Figure Lengend Snippet: Chemical structure of PBDFDTBO and photoluminescent properties of PBDFDTBO and PBDFDTBO-included PEGylated nanoparticles. (A) Chemical structure of PBDFDTBO. (B) UV-VIS spectra of PBDFDTBO in THF solutions at different concentrations, with insert graph showing absorbance peak at 401 nm (blue) and at 574 nm (red) plotted versus the according concentration of PBDFDTBO. (C) Schematic illustration of PEO-PCL-P (left) and PEG-DSPE-P (right) nanoparticle synthesis. (D) Absorption spectra of PEO-PCL-P (red) and PEG-DSPE-P (blue) nanoparticles in aqueous solutions, where absorbance intensity was normalized to the peak absorbance value in each curve. (E) Fluorescence spectra of PEO-PCL-P (red) and PEG-DSPE-P (blue) nanoparticles in aqueous solutions, where fluorescence intensity was normalized to the peak value in each spectrum.

Article Snippet: The size and ζ-potential of PEO-PCL-P or PEG-DSPE-P nanoparticles were measured by Nanoparticle Tracking Analysis (NTA ZetaView ® PMX120) or Zetasizer Nano Analyzer (Malvern ZS90).

Techniques: Concentration Assay, Fluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Differently PEGylated Polymer Nanoparticles for Pancreatic Cancer Delivery: Using a Novel Near-Infrared Emissive and Biodegradable Polymer as the Fluorescence Tracer

doi: 10.3389/fbioe.2021.699610

Figure Lengend Snippet: Optimization of PBDFDTBO encapsulation by differently PEGylated nanoparticles (A) PEG-DSPE-P and (B) PEO-PCL-P. Initial mass ratio between polymer (PEO-PCL or PEG-DSPE) and PBDFDTBO = 1: 0.005, 0.01, 0.02, and 0.04 was applied and the synthesized dye-contained nanoparticles were diluted in PBS with a dilution factor = 2, 5, 10, 20, 50, and 100, respectively, when fluorescence intensity of each solution was measured and plotted versus nanoparticle concentrations.

Article Snippet: The size and ζ-potential of PEO-PCL-P or PEG-DSPE-P nanoparticles were measured by Nanoparticle Tracking Analysis (NTA ZetaView ® PMX120) or Zetasizer Nano Analyzer (Malvern ZS90).

Techniques: Encapsulation, Polymer, Synthesized, Fluorescence

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Differently PEGylated Polymer Nanoparticles for Pancreatic Cancer Delivery: Using a Novel Near-Infrared Emissive and Biodegradable Polymer as the Fluorescence Tracer

doi: 10.3389/fbioe.2021.699610

Figure Lengend Snippet: Physicochemical properties of PEO-PCL-P and PEG-DSPE-P nanoparticles. (A) Cryo-TEM images of both nanoparticles showing sizes of ∼120 nm with a spherical morphology. (B) Size distribution of PEG-DSPE-P (blue) and PEO-PCL-P (red) nanoparticles.

Article Snippet: The size and ζ-potential of PEO-PCL-P or PEG-DSPE-P nanoparticles were measured by Nanoparticle Tracking Analysis (NTA ZetaView ® PMX120) or Zetasizer Nano Analyzer (Malvern ZS90).

Techniques:

Journal: Frontiers in Bioengineering and Biotechnology

Article Title: Differently PEGylated Polymer Nanoparticles for Pancreatic Cancer Delivery: Using a Novel Near-Infrared Emissive and Biodegradable Polymer as the Fluorescence Tracer

doi: 10.3389/fbioe.2021.699610

Figure Lengend Snippet: In vivo profiles of PEO-PCL-P nanoparticles in PANC02 cell-transplanted subcutaneous tumor models after tail-vein injection. (A) Overlapping bright field images and fluorescence images of one representative mouse over time (indicated below the image), depicting the circulation of fluorescent nanoparticles and the accumulation into the observed tumor region (red circle, only showing left flank as example). (B) The fluorescence intensity of mouse blood was plotted versus the withdrawal time, fitting into an exponential function (illustrated as a dotted line in each graph panel), so the exponential decay constant was acquired for conversion into the circulation half-time ( t 1 / 2 ). (C) At 24 h p.i., the mice were sacrificed, and major organs were imaged. (D) The fluorescence intensity of each organ was recorded per tissue area and normalized to that of liver (100%). (E) The fluorescence intensities of both left and right tumors were recorded over time, normalized to the first fluorescence reading in each tumor, and plotted against time. The curves were fitted into linear functions, where the slope represents the accumulation rate of nanoparticles in the tumor.

Article Snippet: The size and ζ-potential of PEO-PCL-P or PEG-DSPE-P nanoparticles were measured by Nanoparticle Tracking Analysis (NTA ZetaView ® PMX120) or Zetasizer Nano Analyzer (Malvern ZS90).

Techniques: In Vivo, Injection, Fluorescence

Journal: Polymers

Article Title: Poly Lactic-co-Glycolic Acid (PLGA) Loaded with a Squaraine Dye as Photosensitizer for Antimicrobial Photodynamic Therapy.

doi: 10.3390/polym16141962

Figure Lengend Snippet: Figure 2. Synthetic workflow for the BrSQ-loaded PLGA nanoparticles preparation via the (a) nanoprecipitation (DoE-1) technique and (b) single emulsion method (DoE-2).

Article Snippet: Nanoparticle Tracking Analyzer (NTA) The mean particle diameter (MPD), polydispersity (PDI), and zeta potential (ζ-potential) of empty and loaded NPs were analyzed through a nanoparticle tracking analyzer (ZetaView® PMX-120 mono-laser, YG-488, Malvern Panalytical, Malvern, UK) equipped with software version 8.05.14_SP7.

Techniques: Emulsion